LOCUS       sequence-54679-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_wt_Venus_195
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        58..167
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             188..205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt ccccggagtt aatccgggac
       61 ctttaattca acccaacaca atatattata gttaaataag aattattatc aaatcatttg
      121 tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac
      181 catggcacac catcaccacc atcacgggag cggggagcag aaactaatat cagaggagga
      241 tcttggctca ggaaccggta gatctgggca catggcggac gaggagaagc tgccgcccgg
      301 ctgggagaag cgcatgagcc gcagctcagg ccgagtgtac tacttcaacc acatcactaa
      361 cgccagccag tgggagcggc ccagcggcaa cagcagcagt ggtggcaaaa acgggcaggg
      421 ggagcctgcc aggaagctgc gcattctgca gtcgactagg gagcaagggc ttgtgggatc
      481 cgtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga agatcaagct
      541 gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg gcgagggcaa
      601 gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc ccctgccctt
      661 ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca ccaagtaccc
      721 cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt gggagcgcac
      781 catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga
      841 ctccttcatc tacgagatac ac
//
