LOCUS       sequence-54710-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_227_Venus_195
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..115
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             136..153
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             163..192
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tccgggacct ttaattcaac ccaacacaat atattatagt taaataagaa ttattatcaa
       61 atcatttgta tattaattaa aatactatac tgtaaattac attttattta caatcaaagg
      121 agatatacca tggcacacca tcaccaccat cacgggagcg gggagcagaa actaatatca
      181 gaggaggatc ttggctcagg aaccggtaga tctgggcaca tggcggacga ggagaagctg
      241 ccgcccggct gggagaagcg catgagccgc agctcaggcc gagtgtacta cttcaaccac
      301 atcactaacg ccagccagtg ggagcggccc agcggcaaca gcagcagtgg tggcaaaaac
      361 gggcaggggg agcctgccag gaagctgcgc attctgcagt cgactaggga gcaagggctt
      421 gtgggatcct ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg
      481 agcaagggcg aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag
      541 atggagggca acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc
      601 tacgacggca ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc
      661 tacgacattc tgaccaccgc gttcgcctac ggc
//
