LOCUS       sequence-54728-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_wt_Venus_195
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(199..711,712..714,715..717))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     392..411
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     651..672
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(775..796)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaccttctg ataggcagcc tgcacctgag gttaatcact tagtgatggt gatggtgatg
       61 gtggtgctcg agtcattaga tatctcattc gtcaagttca agttcttcaa gttttttttg
      121 aaggatatcg gtacccacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      181 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
      241 cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      301 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      361 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      421 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      481 catgtggtcg gggtagcggg cgaagcacat caggccgtag cccagggtgg tcacgagggt
      541 gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca gcttgccgta
      601 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      661 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgcc
      721 accgctgcca cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      781 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
      841 gctcaggtag tggttgtcgg gcagcagcat ggcggccgcg atgtcccgcg cccgcgggaa
      901 ttggaatgaa agctttgcct ttcctactgg tgtacgt
//
