LOCUS       sequence-54738-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_105_Venus_157
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(142..600,601..603,604..606))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     281..300
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     540..561
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(664..685)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaccttctg ataggcagcc tgcacctgag gttaatcact tagtgatggt gatggtgatg
       61 gtggtgctcg agtcattaga tatctcattc gtcaagttca agttcttcaa gttttttttg
      121 aaggatatcg gtacccttgt cggcggtgat atagacgttg tggctgttgt agttgtactc
      181 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      241 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      301 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      361 gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc ccagggtggt
      421 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatcagct tcagggtcag
      481 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
      541 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      601 caccatgcca ccgctgccac ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
      661 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      721 ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      781 gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt
      841 gaagttggcc ttgatgccgt tcttctgcat ggcggccgcg atgtcccgcg cccgcgggaa
      901 ttggaatgaa agctttgcct ttcctactg
//
