LOCUS       sequence-54744-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_105_Venus_229
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..62)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(144..165)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(300..812,813..815,816..818))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     493..512
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     752..773
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttctgatag gcagcctgca cctgaggtta atcacttagt gatggtgatg gtgatggtgg
       61 tgctcgagtc attagatatc tcattcgtca agttcaagtt cttcaagttt tttttgaagg
      121 atatcggtac ccccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      181 ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg
      241 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc
      301 tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg
      361 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
      421 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      481 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
      541 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      601 gcgaagcaca tcaggccgta gcccagggtg gtcacgaggg tgggccaggg cacgggcagc
      661 ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      721 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      781 accaccccgg tgaacagctc ctcgcccttg ctcaccatgc caccgctgcc acccttgtac
      841 agctcgtcca tgccgagagt gatcatggcg gccgcgatgt cccgcgcccg cgggaattgg
      901 aatgaaagct ttgcctttcc tactggtgta cgtggtacgt cag
//
