LOCUS       sequence-54754-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_175_Venus_157
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(139..597,598..600,601..603))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     278..297
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     537..558
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(661..682)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccttctgata ggcagcctgc acctgaggtt aatcacttag tgatggtgat ggtgatggtg
       61 gtgctcgagt cattagatat ctcattcgtc aagttcaagt tcttcaagtt ttttttgaag
      121 gatatcggta cccttgtcgg cggtgatata gacgttgtgg ctgttgtagt tgtactccag
      181 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      241 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      301 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      361 ctgcttcatg tggtcggggt agcgggcgaa gcacatcagg ccgtagccca gggtggtcac
      421 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atcagcttca gggtcagctt
      481 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
      541 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
      601 catgccaccg ctgccaccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      661 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      721 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      781 ctgctggtag tggtcggcga gctgcacgcc gccgtc
//
