LOCUS       sequence-54757-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_175_Venus_195
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..64)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(197..709,710..712,713..715))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     390..409
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     649..670
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(773..794)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accttctgat aggcagcctg cacctgaggt taatcactta gtgatggtga tggtgatggt
       61 ggtgctcgag tcattagata tctcattcgt caagttcaag ttcttcaagt tttttttgaa
      121 ggatatcggt acccacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      181 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      241 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      301 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      361 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      421 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      481 tgtggtcggg gtagcgggcg aagcacatca ggccgtagcc cagggtggtc acgagggtgg
      541 gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg
      601 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      661 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatgccac
      721 cgctgccacc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      781 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
      841 tcaggtagtg gttgtcgggc agcagcatgg cggccgcgat gtcccgcgcc cgcgggaatt
      901 ggaatgaaag ctttgccttt cctactggt
//
