LOCUS       sequence-54786-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_105_Venus_173
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..68)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(243..755,756..758,759..761))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     436..455
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(819..840)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccaccttc tgataggcag cctgcacctg aggttaatca cttagtgatg gtgatggtga
       61 tggtggtgct cgagtcagat atctcattcg tcaagttcaa gttcttcaag ttttttttga
      121 aggatatccc gcgcccgcgg gaattggaat gaaagctttg cctttcctac tggtgtacgt
      181 ggtacgtcag gcgcggtccg ctgtaccgtc gaccactctt cagtcagttg gtcagcggta
      241 ccctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg
      301 gtgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      361 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      421 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      481 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      541 cgggcgaagc acatcaggcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc
      601 agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg
      661 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      721 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgccaccgct gccacccttg
      781 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      841 tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctc
//
