LOCUS       sequence-54792-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_159_Venus_157
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..69)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(250..708,709..711,712..714))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     389..408
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     648..669
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(772..793)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaccacctt ctgataggca gcctgcacct gaggttaatc acttagtgat ggtgatggtg
       61 atggtggtgc tcgagtcaga tatctcattc gtcaagttca agttcttcaa gttttttttg
      121 aaggatatcc cgcgcccgcg ggaattggaa tgaaagcttt gcctttccta ctggtgtacg
      181 tggtacgtca ggcgcggtcc gctgtaccgt cgaccactct tcagtcagtt ggtcagcggt
      241 acccttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      301 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      361 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      421 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      481 gtggtcgggg tagcgggcga agcacatcag gccgtagccc agggtggtca cgagggtggg
      541 ccagggcacg ggcagcttgc cggtggtgca gatcagcttc agggtcagct tgccgtaggt
      601 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      661 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatgccacc
      721 gctgccaccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      781 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
//
