LOCUS       sequence-54796-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_159_Venus_229
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..67)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(254..275)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(410..922,923..925,926..928))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     603..622
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     862..883
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accaccttct gataggcagc ctgcacctga ggttaatcac ttagtgatgg tgatggtgat
       61 ggtggtgctc gagtcagata tctcattcgt caagttcaag ttcttcaagt tttttttgaa
      121 ggatatcccg cgcccgcggg aattggaatg aaagctttgc ctttcctact ggtgtacgtg
      181 gtacgtcagg cgcggtccgc tgtaccgtcg accactcttc agtcagttgg tcagcggtac
      241 ccccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      301 tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      361 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt
      421 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
      481 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      541 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      601 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      661 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcaca
      721 tcaggccgta gcccagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      781 tgcagatcag cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      841 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      901 tgaacagctc ctcgcccttg ctcaccatgc caccgtgcca ccc
//
