LOCUS       sequence-54803-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_175_Venus_229
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(253..274)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     602..621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccaccttctg ataggcagcc tgcacctgag gttaatcact tagtgatggt gatggtgatg
       61 gtggtgctcg agtcagatat ctcattcgtc aagttcaagt tcttcaagtt ttttttgaag
      121 gatatcccgc gcccgcggga attggaatga aagctttgcc tttcctactg gtgtacgtgg
      181 tacgtcaggc gcggtccgct gtaccgtcga ccactcttca gtcagttggt cagcggtacc
      241 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      301 gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      361 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg
      421 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      481 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      541 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      601 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      661 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcacat
      721 caggccgtag cccagggtgg tcacgagggt gggcca
//
