LOCUS       sequence-54809-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Kon T, Oyama T, Shimo-Kon R, Imamula K, Shima T, Sutoh K, Kurisu G
  TITLE     The 2.8 A crystal structure of the dynein motor domain.
  JOURNAL   Nature. 2012 Mar 7;484(7394):345-50. doi: 10.1038/nature10955.
  PUBMED    22398446
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "484"; issue: "7394"; pages:
            "345-50"
COMMENT     Sequence Label: pMB38-HF380deltaMTBD
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(478..501)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(511..528)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgagagtt gtccaaaatt ctctaacacc tttaaggaat tcctctaaag caatttcacc
       61 ttgagctgca gtgataacct cacgatagat attttcattt cttgctaaat ctgaatccca
      121 aatggaaccc aatgttaact ctgtaatgat ccaattggta tttaaacgtt tctttaaaat
      181 tttccaatga cgatctttga tggcttcaga gtgaagatcg gtgatgattg cattaccttt
      241 gagatagatt ttaattaagt tttgagcatg atcaaaggca gagtattgac gaatacgatt
      301 tggtaaattt ttcaattttt gtaaagtatc ttccaatgat tttctaactt ttcttggaat
      361 gatagcagac caagcagtct ctttaagact atcgatttct tgccatgtat ttgaaagttc
      421 gacccaaact gctttcaaat cttgaatctc ctcttcgact ggtaccttac caccaccttt
      481 atcatcatca tctttataat caccaccacc atgatgatga tgatgatgac gcgtcatttt
      541 tagatctgat ttttattttt tatttaattt aatttatttg ttttaagaaa taagaaaaaa
      601 aaaaaaaaaa a
//
