LOCUS       sequence-54812-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tandem dimer of pea ascorbate peroxidase (W41F mutant). This 
            construct has been replaced by APEX2 (construct 49386). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 tdAPX W41F
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atagaatagg gccctctaga tgcatgctcg agttatcact ttatcgtcgt catccttgta
       61 gtcggcttca gcaaatccaa gctcagagag cttaagatgt gcttcagcat aatcagcaaa
      121 gaaaacatct tcatccgcag catatttctc aacaagaggg cggaatacag agtcagtcaa
      181 aagtgcctta tcacttggca actgaagaag gccatccttc tcaccagtca acaactcagt
      241 gaaatatgag ttgtcaaaaa tgagaggatt agaagtccac ggtccctcaa atccagaacg
      301 ctccttgtgt gcagctccaa tggtgtgacc accagataga gcaacaatgt cctgatcact
      361 aagccccata gcctttccaa acacatccct caaatggtca gaacccttag tggcatcagg
      421 caagcgaccc tcaggtggtg gctcaggctt gtcctcccta ccagggtgga aaggaacttc
      481 aggtccaccg gtaatctcaa cagcaacaac accagccaac tggtagaaat cagcatagct
      541 cacaataggg aattgctcct taataggctc caacagccta accgcgatat caagaccgtt
      601 gttagcacca tgagcaagct cagcttggtg cttaattgtt ccgaaaggac caccagtctt
      661 tgtcttggaa tcaaaagtac cagcagagtg gaatgccaaa cggagaatta gaggagcgca
      721 tttcttctca gcgataaaac ctctgagctt cctcttagcc ttttcaatgg ccttctggta
      781 atcgggacta acagttgggg taagattttc cccttggatc cttgctcccg gccagtcccg
      841 ccagaacctt tggtagagcc ttccccggag cccggtttgc cagaaccaga ggtggagcca
      901 gcctcggcga agcccagctc
//
