LOCUS       sequence-54816-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_227_Venus_157
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(244..702,703..705,706..708))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     383..402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     642..663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(766..787)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccttctgata ggcagcctgc acctgaggtt aatcacttag tgatggtgat ggtgatggtg
       61 gtgctcgagt cagatatctc attcgtcaag ttcaagttct tcaagttttt tttgaaggat
      121 atcccgcgcc cgcgggaatt ggaatgaaag ctttgccttt cctactggtg tacgtggtac
      181 gtcaggcgcg gtccgctgta ccgtcgacca ctcttcagtc agttggtcag cggtaccctt
      241 gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      301 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      361 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      421 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      481 ggggtagcgg gcgaagcaca tcaggccgta gcccagggtg gtcacgaggg tgggccaggg
      541 cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc
      601 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      661 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgc caccgctgcc
      721 acccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      781 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      841 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      901 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttga
//
