LOCUS       sequence-54817-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_227_Venus_173
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(join(238..750,751..753,754..756))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     431..450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(814..835)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccttctgata ggcagcctgc acctgaggtt aatcacttag tgatggtgat ggtgatggtg
       61 gtgctcgagt cagatatctc attcgtcaag ttcaagttct tcaagttttt tttgaaggat
      121 atcccgcgcc cgcgggaatt ggaatgaaag ctttgccttt cctactggtg tacgtggtac
      181 gtcaggcgcg gtccgctgta ccgtcgacca ctcttcagtc agttggtcag cggtaccctc
      241 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      301 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      361 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      421 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      481 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
      541 gaagcacatc aggccgtagc ccagggtggt cacgagggtg ggccagggca cgggcagctt
      601 gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      661 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      721 caccccggtg aacagctcct cgcccttgct caccatgcca ccgctgccac ccttgtacag
      781 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      841 gcgcttctcg ttggggtctt tgctcagg
//
