LOCUS       sequence-54819-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA
  TITLE     Sequence- and structure-specific RNA processing by a CRISPR 
            endonuclease.
  JOURNAL   Science. 2010 Sep 10;329(5997):1355-8.
  PUBMED    20829488
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-09-10"; volume: "329(5997)"; pages: "1355-8"
COMMENT     Sequence Label: pHMGWA-Pa14Csy4H29A
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..564
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLAQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
ORIGIN
        1 atggaccact acctcgacat tcgcttgcga ccggacccgg aatttccccc ggcgcaactc
       61 atgagcgtgc tcttcggcaa gctcgcccag gccctggtgg cacagggcgg ggacaggatc
      121 ggcgtgagct tccccgacct cgacgaaagc cgctcccggc tgggcgagcg cctgcgcatt
      181 catgcctcgg cggacgacct tcgtgccctg ctcgcccggc cctggctgga agggttgcgg
      241 gaccatctgc aattcggaga accggcagtc gtgcctcacc ccacaccgta ccgtcaggtc
      301 agtcgggttc aggcgaaaag caatccggaa cgcctgcggc ggcggctcat gcgccggcac
      361 gatctgagtg aggaggaggc tcggaaacgc attcccgata cggtcgcgag agccttggac
      421 ctgcccttcg tcacgctacg cagccagagc accggacagc acttccgtct cttcatccgc
      481 cacgggccgt tgcaggtgac ggcagaggaa ggaggattca cctgttacgg gttgagcaaa
      541 ggaggtttcg ttccctggtt ctga
//
