LOCUS       sequence-54825-e        3185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Wayne N, Bolon DN
  TITLE     Dimerization of Hsp90 is required for in vivo function. Design and 
            analysis of monomers and dimers.
  JOURNAL   J Biol Chem. 2007 Nov 30;282(48):35386-95. Epub 2007 Oct 1.
  PUBMED    17908693
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-11-30"; volume: "282(48)"; pages: "35386-95. 
            Epub 2007 Oct 1"
COMMENT     Sequence Label: pRNDM
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            complement(100..1462)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(100..297)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(303..1118)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMRLALPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1032..1051
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1119..1462)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(1515..1533)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1516..1533)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1755..1774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1874..1896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1934..1952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1993..2496
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     rep_origin      2579..3167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3068..3087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctgggaac aaaagctgga gctaaggcgc gccggtttaa acgagctcgt
       61 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      121 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      181 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      241 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      301 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      361 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      421 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
      481 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
      541 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
      601 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
      661 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
      721 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
      781 tattcttcta atacctggaa tgctgttttg ccggggatcg cagtggtgag taaccatgca
      841 tcatcaggag tacggataaa atgcttgatg gtcggaagag ctaatcgcat aaattccgtc
      901 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
      961 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     1021 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     1081 aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgg ttgtttatgt tcggatgtga
     1141 tgtgagaact gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg
     1201 gcaaatccta accttttata tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt
     1261 ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc
     1321 gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct
     1381 aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga
     1441 gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag gcaagctaaa
     1501 cagatcggcc gcgttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     1561 tattaattgt agccgcgttc taacgacaat atgtccatat atgcgtatat ataccaatct
     1621 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     1681 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta
     1741 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     1801 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     1861 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     1921 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1981 gtttcttaga cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga
     2041 ataatttggg aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa
     2101 agtaaataaa gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa
     2161 aaaatttcaa caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa
     2221 atagatatac attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg
     2281 tcttctacac agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag
     2341 ataaaaggta gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa
     2401 actatttttt ctttaatttc tttttttact ttctattttt aatttatata tttatattaa
     2461 aaaatttaaa ttataattat ttttatagca cgtgatgaaa aggacccagg tggcactttt
     2521 cggggaaatg tgcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3181 cggcc
//
