LOCUS       sequence-54999-e        2430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TAL3186
FEATURES             Location/Qualifiers
     source          1..2430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..84
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             91..111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctagagcta gcaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
       61 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
      121 cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca acaggagaaa
      181 atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt ggggcatggc
      241 ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg gacggtggct
      301 gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc aattgtaggg
      361 gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag
      421 cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc gaagagaggg
      481 ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg ggcccccttg
      541 aacctgaccc cagaccaggt agtcgcaatc gcgtcacatg acgggggaaa gcaagccctg
      601 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa
      661 gtcgtggcca ttgcaaataa taacggtggc aaacaggctc ttgagacggt tcagagactt
      721 ctcccagttc tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg
      781 aacattggag ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa
      841 gcccacggtt tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag
      901 gcgctggaaa cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca
      961 gaccaggtag tcgcaatcgc gtcgaacatt gggggaaagc aagccctgga aaccgtgcaa
     1021 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt
     1081 gcaagcaaca tcggtggcaa acaggctctt gagacggttc agagacttct cccagttctc
     1141 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg
     1201 aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg
     1261 acgcctgcac aagtggtcgc catcgccaac aacaacggcg gtaagcaggc gctggaaaca
     1321 gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc
     1381 gcaatcgcgt cgaacattgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg
     1441 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaatggg
     1501 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac
     1561 gggctgactc ccgatcaagt tgtagcgatt gcgaataaca atggagggaa acaagcattg
     1621 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa
     1681 gtggtcgcca tcgcctcgaa tggcggcggt aagcaggcgc tggaaacagt acagcgcctg
     1741 ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgtca
     1801 aacggagggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa
     1861 gaccacggcc ttacaccgga gcaagtcgtg gccattgcat cccacgacgg tggcaaacag
     1921 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc
     1981 gatcaagttg tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa
     2041 cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc
     2101 gccagccatg atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg
     2161 tgccaggatc atggactgac acccgaacag gtggtcgcca ttgcttccca cgacggagga
     2221 cggccagcct tggagtccat cgtagcccaa ttgtccaggc ccgatcccgc gttggctgcg
     2281 ttaacgaatg accatctggt ggcgttggca tgtcttggtg gacgacccgc gctcgatgca
     2341 gtcaaaaagg gtctgcctca tgctcccgca ttgatcaaaa gaaccaaccg gcggattccc
     2401 gagagaactt cccatcgagt cgcgggatcc
//
