LOCUS       sequence-55-expo        5087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD30_69
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2661..2683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2783..2802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2880..2898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2966..3070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3071..3139,3140..3931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3289..3308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4102..4690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4591..4610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4844..4861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4978..4999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5014..5019,5020..5037,5038..5044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5052..5068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5057..5079
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(5076..5087,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(5076..5087,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccagctt
       61 gcatgcctgc aggtcgaggc atttgaattg ggggtggtgg acagtaactg tctgtaataa
      121 taattactcc tgaccaggtt gcaattcgag ttttgataag cataattata ccttgtacat
      181 tgtgggtttt gtgctgtgga cgttttattg tgacatcccc ataagctaca agaacaaaaa
      241 tgaaatgtaa aagtattgaa aacgtcgtac attttatatc tgagtagtat cctttgcttt
      301 aaatgtccat aaaactaatt ttataatcaa taaaacaacg tttgtaaatc aactgagttt
      361 acaagtagag acattgaggg atactttcac tatgctaaag tgataatcga ccaaataata
      421 acttcacttt ggtatttatt cctgtcttat aaatgttatg tatgaattaa attcatatgc
      481 atatggctca ctctgacaat ttttaataat cttccagatc aatattgact accgatgcgg
      541 gtggtctttt gctttgaatt ctgctgaact ttacaccccg aacagcaatg tgtgcttcag
      601 cctaaaaaaa agtaagtgtg ttaatcagtg cccccgattc ttcatttttt gcccctctct
      661 cccgtttcgt cggcaaaaga agagaaaata aagataagtc tcaagatagg ttggtaatcg
      721 ctaaagtggt tgtgtggata agagtagcaa aatggcagga agagcacttt gcgcgcacac
      781 actgtactca ttgttctgga taaaattctc tcgttgtttg ccgtcggatg tctgcctctc
      841 tgcattgagc cggcttcttc actatcttta gttaacctaa aatgccgttt cttttctcgt
      901 atccccacta tcccgttgag gttctctgct ctcttcgctc cctaccgcca gcgagcaact
      961 atccgtgggg gcgccttgct cggaagatgg gggggaagaa agaagatttt tgctatttgc
     1021 acttgagaaa gagacttttc ctgcgtcgat ggttagagaa cagtgtgcag acacttttca
     1081 gctacctaga attacaattg gatatccccg cctcccaatc cacccaccca gggaaaaaga
     1141 agggctcgcc gaaaatcaaa gttatctcca ggctcgcgca tcccaccgag cggttgactt
     1201 ctctccacca cttttcattt taaccctcgg ggtacgggat tggccaaagg acccaaaggt
     1261 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc
     1321 gacggtacca tggtattgat atctgagctc cgcatcggcc gctgtcatca gatcgccatc
     1381 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
     1441 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     1501 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     1561 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     1621 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     1681 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     1741 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     1801 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     1861 ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac
     1921 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     1981 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     2041 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     2101 gtttaaaatt aaacattttt ctaaatttta tatgatttct tttaaatttg caaaaattac
     2161 ttaaatttga attcccgcgc aaatgagtga cttcattttc tgcattattg tgttttccgg
     2221 ctatattaat aggtatttgt ttgtgttttt ctttatttta tgattcgaac atatttccct
     2281 aaagaaaaaa tatgattaat ctggaaaaat tggaaaatta tttttcaaat aaaaaacaaa
     2341 gaaaaaaatg aagaaaaacc tattagtttg gccataaaac gcaaaaatgt cgaaaatgac
     2401 gtcactcatc tgcgcgggaa atcaagaata attcggcctt ttttattttt ttggaaaatc
     2461 gtaaaacatt tagaaaaatt ttttaatagt tatatgggac ttattctgca tttagggcaa
     2521 aagccagaga ctactccacc gttaacatga attatgaata ttattgcgac aagacccaaa
     2581 cattgataaa cccaaatcta gcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     2641 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2701 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2761 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     2821 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     2881 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2941 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3001 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3061 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3121 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3181 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3241 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3301 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3361 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3421 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3481 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3541 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3601 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3661 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3721 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3781 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3841 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3901 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3961 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4021 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4081 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     4501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4681 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4741 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4801 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4861 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4921 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4981 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5041 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagct
//
