LOCUS       sequence-55021-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     hSyn1-FLEX-hChR2-tdtomato
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSyn1-FLEX-hChR2-tdtomato
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..103
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtcgatggt gagatctgga ctagagggtc gatggtgatg cttggatcaa aataggaaga
       61 atcccaatga taacttcgta taaagtatcc tatacgaagt tatactagta gtacgttggc
      121 cggccactag aattcggtac cgcgggcccc tagcgccacc atggactatg gcggcgcttt
      181 gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct gtggtggtga acgggtccgt
      241 cctggtccct gaggatcaat gttactgtgc cggatggatt gaatctcgcg gcacgaacgg
      301 cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca ggattcagca ttttgctgct
      361 gatgttctat gcctaccaaa cctggaaatc tacatgcggc tgggaggaga tctatgtgtg
      421 cgccattgaa atggttaagg tgattctcga gttctttttt gagtttaaga atccctctat
      481 gctctacctt gccacaggac accgggtgca gtggctgcgc tatgcagagt ggctgctcac
      541 ttgtcctgtc atccttatcc acctgagcaa cctcaccggc ctgagcaacg actacagcag
      601 gagaaccatg ggactccttg tctcagacat cgggactatc gtgtgggggg ctaccagcgc
      661 catggcaacc ggctatgtta aagtcatctt cttttgtctt ggattgtgct atggcgcgaa
      721 cacatttttt cacgccgcca aagcatatat cgagggttat catactgtgc caaagggtcg
      781 gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc gtgagctggg gtatgttcc
//
