LOCUS       sequence-55022-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     hSyn1-FLEX-hChR2-tdtomato
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSyn1-FLEX-hChR2-tdtomato
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      432..441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(493..526)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(597..630)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(725..745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtagtcgttg ctcaggccgg tgaggttgct caggtggata aggatgacag gacaagtgag
       61 cagccactct gcatagcgca gccactgcac ccggtgtcct gtggcaaggt agagcataga
      121 gggattctta aactcaaaaa agaactcgag aatcacctta accatttcaa tggcgcacac
      181 atagatctcc tcccagccgc atgtagattt ccaggtttgg taggcataga acatcagcag
      241 caaaatgctg aatcctgctg caagccactg caggacattt gacgcggtct gagcgccgtt
      301 cgtgccgcga gattcaatcc atccggcaca gtaacattga tcctcaggga ccaggacgga
      361 cccgttcacc accacaggat tagtaacgaa caaaagttcg cgtccgacgg cagacaaagc
      421 gccgccatag tccatggtgg cgctaggggc ccgcggtacc gaattctagt ggccggccaa
      481 cgtactacta gtataacttc gtataggata ctttatacga agttatcatt gggattcttc
      541 ctattttgat ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa
      601 cttcgtatag catacattat acgaagttat gtccctcgaa gaggttcgaa ttcgatatca
      661 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
      721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
      781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
      841 atgag
//
