LOCUS       sequence-55026-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Rodriguez-Viciana P, Warne PH, Vanhaesebroeck B, Waterfield MD, 
            Downward J
  TITLE     Activation of phosphoinositide 3-kinase by interaction with Ras and 
            by point mutation.
  JOURNAL   EMBO J. 1996 May 15;15(10):2442-51.
  PUBMED    8665852
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1996-05-15"; volume: "15(10)"; pages: "2442-51"
COMMENT     Sequence Label: pcDNA3-myc-p110-alpha(K227E)
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aggcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccgagctcgg atccgccacc atggaacaga
      121 aactcatatc ggaggaggat ctacctccaa gaccatcatc aggtgaactg tggggcatcc
      181 acttgatgcc cccaagaatc ctagtagaat gtttactacc aaatgggatg atagtgactt
      241 tagaatgcct ccgtgaggct acgttaataa cgataaagca tgaactattt aaagaagcaa
      301 gaaaataccc tctccatcaa cttcttcaag atgaatcttc ttacattttc gtaagtgtta
      361 cccaagaagc agaaagggaa gaattttttg atgaaacaag acgactttgt gaccttcggc
      421 tttttcaacc ctttttaaaa gtaattgaac cagtaggcaa ccgtgaagaa aagatcctca
      481 atcgagaaat tggttttgct atcggcatgc cagtgtgtga attcgatatg gttaaagatc
      541 cagaagtaca ggacttccga agaaatattc tcaatgtttg taaagaagct gtggatctta
      601 gggatcttaa ttcacctcat agtagagcaa tgtatgttta tcctccaaat gtagaatctt
      661 caccagaact gccaaagcac atatataata aattggataa agggcaaata atagtggtga
      721 tttgggtaat agtttctcca aataatgaca aacagaagta tactctgaaa atcaaccatg
      781 actgtgtgcc agaacaagta attgctgaag caatcaggga aaaaacgcgt agtatgttgc
      841 tatcatctga acaactaaaa ctctgtgttt tagaatatca gggcaagtat attttaaaag
      901 tgtgtggatg tgatgaatac ttcctagaaa atatcctctg agtcagtata agtatataga
      961 agctgtataa
//
