LOCUS       sequence-55027-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pTriExRhoA2G
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             607..630
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(720..739)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    785..840
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(839..858)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gagctgtaca agggtaccgc tgccattcgt aagaaactgg tgattgttgg tgatggagcc
       61 tgtggaaaga catgcttgct catagtcttc agcaaggacc agttcccaga ggtgtatgtg
      121 cccacagtgt ttgagaacta tgtggcagat atcgaggtgg atggaaagca ggtagagttg
      181 gctttgtggg acacagctgg gcaggaagat tatgatcgcc tgaggcccct ctcctaccca
      241 gataccgatg ttatactgat gtgtttttcc atcgacagcc ctgatagttt agaaaacatc
      301 ccagaaaagt ggaccccaga agtcaagcat ttctgtccca acgtgcccat catcctggtt
      361 gggaataaga aggatcttcg gaatgatgag cacacaaggc gggagctagc caagatgaag
      421 caggagccgg tgaaacctga agaaggcaga gatatggcaa acaggattgg cgcttttggg
      481 tacatggagt gttcagcaaa gaccaaagat ggagtgagag aggtttttga aatggctacg
      541 agagctgctc tgcaagctag acgtgggaag aaaaaatctg gttgccttgt cttgtgataa
      601 ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct
      661 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
      721 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
      781 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
      841 ctcggaagga catatgggag ggcaaatcat ttaaaacatc a
//
