LOCUS       sequence-55028-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiEKAR2G1
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..82)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(152..886)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     345..364
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     604..625
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(728..749)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgacg aattcatatg ctagctctag actgcaggat
       61 cctggtgatg gtgatggtgg tgctcgagtc attagatatc tcattcgtca agttcaagtt
      121 cttcaagttt tttttgaagg atatcggtac cctcgatgtt gtggcggatc ttgaagttgg
      181 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg ttgtagttgt
      241 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      301 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      361 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      421 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagcccaggg
      481 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc agcttcaggg
      541 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      601 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      661 tgctcaccat gccaccgctg ccacccttgt acagctcgtc catgccgaga gtgatcccgg
      721 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      781 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      841 gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc gtccatcgcg gccgcgatgt
      901 cccgcgcccg cgggaattgg aatgaaagct ttgccttt
//
