LOCUS       sequence-55031-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiRhoA2G
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..82)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(677..928)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(719..740)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgacg aattcatatg ctagctctag actgcaggat
       61 cctggtgatg gtgatggtgg tgctcgagtt atcacaagac aaggcaacca gattttttct
      121 tcccacgtct agcttgcaga gcagctctcg tagccatttc aaaaacctct ctcactccat
      181 ctttggtctt tgctgaacac tccatgtacc caaaagcgcc aatcctgttt gccatatctc
      241 tgccttcttc aggtttcacc ggctcctgct tcatcttggc tagctcccgc cttgtgtgct
      301 catcattccg aagatccttc ttattcccaa ccaggatgat gggcacgttg ggacagaaat
      361 gcttgacttc tggggtccac ttttctggga tgttttctaa actatcaggg ctgtcgatgg
      421 aaaaacacat cagtataaca tcggtatctg ggtaggagag gggcctcagg cgatcataat
      481 cttcctgccc agctgtgtcc cacaaagcca actctacctg ctttccatcc acctcgatat
      541 ctgccacata gttctcaaac actgtgggca catacacctc tgggaactgg tccttgctga
      601 agactatgag caagcatgtc tttccacagg ctccatcacc aacaatcacc agtttcttac
      661 gaatggcagc ggtacccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      721 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      781 ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      841 gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt
      901 tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgttgtagt
      961 tgtactccag cttgtgcccc agga
//
