LOCUS       sequence-55037-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEXV3-Myc-H-RAS_V12
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(291..317)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          371..467
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(506..835)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      520..655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     820..840
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(910..928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tactggtgga tgtcctcaaa agacttggtg ttgttgatgg caaacacaca caggaagccc
       61 tccccggtgc gcatgtactg gtcccgcatg gcgctgtact cctcctggcc ggcggtatcc
      121 aggatgtcca acaggcacgt ctccccatca atgaccacct gcttccggta ggaatcctct
      181 atagtggggt cgtattcgtc cacaaaatgg ttctggatca gctggatggt cagcgcactc
      241 ttgcccacac cgacggcgcc aacaacaaca agcttatatt ccgttggtgg agcgtaatct
      301 ggaacatcgt atgggtacat cgctccgaat tccgcagctt ttagagcaga agtaacactt
      361 ccgtacaggc ctagaagtaa aggcaacatc cactgaggag cagttctttg atttgcacca
      421 ccaccggatc cgggacctga aataaaagac aaaaagacta aacttaccag ttaactttct
      481 ggtttttcag ttcctcgagg agctttttgc aaaagcctag gcctccaaaa aagcctcctc
      541 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
      601 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
      661 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
      721 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
      781 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
      841 acagccaagc ttatcgatga taagctgtca aacatgagaa ttggtcgacc aattcttgaa
      901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatgtttc
      961 ttagacgtca gtg
//
