LOCUS       sequence-55052-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral backbone for cloning and gene expression. Select with 
            puromycin or GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pMSCV PIG (Puro IRES GFP empty vector)
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..581
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    31..598
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(191..210)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     420..437
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcgcccttgc tcaccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac
       61 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca
      121 tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag
      181 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac
      241 aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc
      301 gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt
      361 tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg
      421 cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg
      481 tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa
      541 cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg ggggcggaat
      601 tggatgcatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc
      661 aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg
      721 cggtgacggt gaagccga
//
