LOCUS       sequence-55058-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, Ahn NG
  TITLE     Constitutive activation of extracellular signal-regulated kinase 2 
            by synergistic point mutations.
  JOURNAL   J Biol Chem. 2001 Dec 7;276(49):46469-79. Epub 2001 Oct 8.
  PUBMED    11591711
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-12-7"; volume: "276(49)"; pages: "46469-79. Epub
            2001 Oct 8"
COMMENT     Sequence Label: pCMV5-rat ERK2-D319N
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             292..327
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     primer_bind     complement(557..576)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 ctgaattgta taataaattt aaaagctaga aactatttgc tttctctccc gcacaaaaat
       61 aaggtgccgt ggaacagatt gttcccaaac gctgactcca aagctctgga tttactggat
      121 aaaatgttga catttaaccc tcacaagagg attgaagttg aacaggctct ggcccacccg
      181 tacctcgagc agtattatga cccaagtaat gagcccattg ctgaagcacc attcaagttt
      241 gacatggagc tggacgactt acctaaggag aagctcaaag aactcatttt tgaagagact
      301 gctcgattcc agccaggata cagatcttaa caagcggccg cgactctaga ggatcccggg
      361 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
      421 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
      481 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
      541 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
      601 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
      661 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
      721 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
      781 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt c
//
