LOCUS       sequence-55063-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Lubelski D, Ponzio TA, Gainer H
  TITLE     Effects of A-CREB, a dominant negative inhibitor of CREB, on the 
            expression of c-fos and other immediate early genes in the rat SON 
            during hyperosmotic stimulation in vivo.
  JOURNAL   Brain Res. 2012 Jan 6;1429:18-28. Epub 2011 Oct 26.
  PUBMED    22079318
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain 
            Res."; date: "2012-01-6"; volume: "1429"; pages: "18-28. Epub 2011 
            Oct 26"
COMMENT     Sequence Label: pCMV-EGFP-T2A-ACREB-WPRE
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..79
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(409..429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(827..838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctcggcatg gacgagctgt acaaggaggg cagaggaagt cttctaacat gcggtgacgt
       61 ggaggagaat cccggccctg aattcatgga ctacaaggac gacgatgaca agcatatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatcctgac ctggaacaac gtgctgagga
      181 actggcccgt gaaaacgaag agctggaaaa agaggccgaa gagctggagc aggaactggc
      241 agaactcgag aacagagtgg cagtgcttga aaaccaaaac aaaacattga ttgaggagct
      301 aaaagcactt aaggaccttt actgccacaa gtcagattaa ttcaagcttg tcgacaatca
      361 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
      421 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
      481 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
      541 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
      601 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
      661 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
      721 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
      781 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
      841 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
      901 tcgccctcag acga
//
