LOCUS       sequence-55078-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72110-BBa_J72097
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     131..148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(181..208)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(224..295)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(560..678)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
ORIGIN
        1 aggcctcccc tgattctgtg gataaccggg atctgtaagg atcaaccact ttgtacaaga
       61 aagctgggtc gaattgatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      121 gaccgagcgc agcgagtcag tgagcgagga agcctgcacg tcgagttagg atcctataaa
      181 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
      241 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      301 tggagatcct tattgtgagt ctggctggtt agtggccatg agatattcga ttgtgtcagt
      361 gagatcatcc aggtcgtctt gggtgatgcg gtactcctga ttggatatct ttgagtagtg
      421 ttcagcaatg gcgcgggcag cgtcggtttc ggcggggtct acagataaag cgttagagca
      481 atgtctaacg tcgtcgatgg ttggtggaat gaaagccata attatgcctc actgtaagat
      541 ccatcctgct gaatttcatt acgaccagtc taaaaagcgc ctgtattcgc gaccttctcg
      601 ttactgacag gaaaatgggc cattggcaac cagggaaaga tgaacgtgat gatgttcaca
      661 atttgctgaa ttgtggtgat gtgatgctca ccgcatttcc tgaaaattca cgctgtatct
      721 tgaaaaatcg acgtttttta cgtggttttc cgtcgaaaat ttaaggtaag aacctgacct
      781 cgtgattact atttcgccgt gttgacgaca tcaggaggcc agtatgaccg tat
//
