LOCUS       sequence-55081-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Parikh MR, Greene DN, Woods KK, Matsumura I
  TITLE     Directed evolution of RuBisCO hypermorphs through genetic selection 
            in engineered E.coli.
  JOURNAL   Protein Eng Des Sel. 2006 Mar;19(3):113-9. Epub 2006 Jan 19.
  PUBMED    16423843
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel."; date: "2006-03"; volume: "19(3)"; pages: "113-9. Epub 
            2006 Jan 19"
COMMENT     Sequence Label: PBAD-his6-prkA-pACYC184
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..110
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             114..146
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accatgggca
       61 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta
      121 gcatgactgg tggacagcaa atgggtcgcg gatccgaatt cgatcccgag ctcgtcactg
      181 tctcgaggga tcctatgagc aagccagatc gtgttgtttt gatcggcgtt gccggtgact
      241 ccggttgcgg caaatcaacc ttcctaaatc gccttgccga cttgtttggt acggaattga
      301 tgacggtcat ctgcttggat gactatcaca gtctcgatcg caagggccgg aaggaagcag
      361 gcgtaacggc tttggatccc cgcgccaaca actttgactt gatgtatgaa caggtcaagg
      421 cgttgaagaa cggcgaaacg atcatgaagc cgatctacaa ccatgaaacc ggcttgatcg
      481 atccgcccga aaaaatcgaa cccaatcgca tcattgtgat cgagggtctg catccgcttt
      541 acgacgagcg cgtgcgtgaa ctgctcgatt tcagcgttta cctcgacatc gatgacgaag
      601 tcaaaatcgc ttggaagatc caacgcgata tggcagaacg cggccactcc tacgaagatg
      661 tcctcgcctc gatcgaagcg cgccgccctg acttcaaggc ctacattgag ccccagcgtg
      721 gccatgcgga catcgtcatc cgcgtcatgc cgacccagct aatccc
//
