LOCUS       sequence-55103-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_wt_Venus_157
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        56..165
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             186..203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             213..242
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgactcact ataggggaat tgtgagcgga taacaattcc ccggagttaa tccgggacct
       61 ttaattcaac ccaacacaat atattatagt taaataagaa ttattatcaa atcatttgta
      121 tattaattaa aatactatac tgtaaattac attttattta caatcaaagg agatatacca
      181 tggcacacca tcaccaccac cacgggagcg gggagcagaa actaatatca gaggaggatc
      241 ttggctcagg ttcaggatcc gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc
      301 ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg
      361 agggcgaggg cgagggcaag ccctacgacg gcaccaacac catcaacctg gaggtgaagg
      421 agggagcccc cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca
      481 gggccttcac caagtacccc gacgacatcc ccaactactt caagcagtcc ttccccgagg
      541 gctactcttg ggagcgcacc atgaccttcg aggacaaggg catcgtgaag gtgaagtccg
      601 acatctccat ggaggaggac tccttcatct acgagataca cctcaagggc gagaacttcc
      661 cccccaacgg ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc accgagagga
      721 tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca caagctgctg ctggagggcg
      781 gcggccacca ccgcgttgac ttcaagacca tctacagggc caagaaggcg gtgaagctgc
      841 ccgactatca ctttgtggac caccgcatcg agatcctgaa ccacgacaag gactacaaca
      901 aggtgaccgt ttacgagagc gccgtggccc gcaactccac cgacggcatg
//
