LOCUS       sequence-55115-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW
  TITLE     Sds22 and Repo-Man stabilize chromosome segregation by counteracting
            Aurora B on anaphase kinetochores.
  JOURNAL   J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.
  PUBMED    22801782
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-07-23"; volume: "198(2)"; pages: "173-83. Epub 
            2012 Jul 16"
COMMENT     Sequence Label: pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-pu...
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(260..262,263..721)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(302..323)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(563..582)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaaattcctt ttacaagacg gagatgaaat caagatcatt tgggataaaa acaataaatt
       61 tgtcattggc tttaaagtgg aaattaacga tactacaggt ctgtttaacg agggattagg
      121 tatgttacaa gaacaaagag tagtacttaa gcaaacagcc gaagaaaaag atttggtgaa
      181 aaagttagga ggatccggcg gcaaggtgaa caagatcgtg aagaaccgcc gcaccgtggc
      241 catcggtggt tccggaggag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      301 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      361 gggcgatgcc acctacggca agctgaccct gaagctgctc tgcaccaccg gcaagctgcc
      421 cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta
      481 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      541 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      601 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      661 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc
      721 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cg
//
