LOCUS       sequence-55120-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW
  TITLE     Sds22 and Repo-Man stabilize chromosome segregation by counteracting
            Aurora B on anaphase kinetochores.
  JOURNAL   J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.
  PUBMED    22801782
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-07-23"; volume: "198(2)"; pages: "173-83. Epub 
            2012 Jul 16"
COMMENT     Sequence Label: pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-pu...
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(162..872,873..875,876..878))
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(join(162..872,873..875,876..878))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(join(162..872,873..875,876..878))
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(204..225)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     553..572
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgaactcgag acaacctact gtcttcaatt ttacaattgc aatcctcgga tctaccaatg
       61 aaaaatggat tcacaccttg ctgaatctcc aggctttctt gaataatgct gtcaggcaat
      121 ggttttaaag ttaaaaacct accattaccc ttggtaccag ccttgtacag ctcgtccatg
      181 ccgtgagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      241 ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg caggatcacg
      301 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
      361 gtgatgttgt ggcgggcctt gaagtgggcc ttgatgccgt tcttctgctt gtcggcggtg
      421 atatagacgt tgtggctgat gtagttgtac tccagcttgt gccccaggat gttgccgtcc
      481 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      541 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtacg ctcctggacg
      601 tagccttcgg gcatgacgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      661 ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
      721 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      781 tcgccggaca cgctgaacct gtggccgttt acgtcgccct ccagctcgac caggatgggc
      841 acgatcccgc cgaacagctc ctcgcccttg ctcaccatgg tggcgaagct tgctagctta
      901 gcgctggtgt a
//
