LOCUS       sequence-55121-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW
  TITLE     Sds22 and Repo-Man stabilize chromosome segregation by counteracting
            Aurora B on anaphase kinetochores.
  JOURNAL   J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.
  PUBMED    22801782
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-07-23"; volume: "198(2)"; pages: "173-83. Epub 
            2012 Jul 16"
COMMENT     Sequence Label: pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-pu...
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..466
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      479..488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(791..810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggc gcgccaccat gccagagcca gcgaagtctg ctcccgcccc
      121 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
      181 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
      241 tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt
      301 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
      361 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
      421 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaagctag caagcttcgc
      481 caccatggtg agcaagggcg aggagctgtt cggcgggatc gtgcccatcc tggtcgagct
      541 ggagggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac
      601 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      661 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
      721 gaagcagcac gacttcttca agtccgtcat gcccgaaggc tacgtccagg agcgtaccat
      781 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      841 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      901 gcacaagctg gag
//
