LOCUS       sequence-55123-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to EYFP driven by human Synapsin I promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Apr 5. 446(7136):633-9."
COMMENT     Sequence Label: pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcaccatg gtggcggccg ctggcacggc tccggcctcg gcttcgtctt cgacgagagt
       61 ctcgacctcg atctccgttc cgccgatgtt cagtttggtg gttttgcgga tatctccgtg
      121 aatcaatatg tgctcgtgga tcaggacgcg caggtagtgt cccaacaacc cccaacaatt
      181 tttactcatc agatcaataa tcgtgtgacc tacggtggag ccatagacgc tcaggacgcc
      241 aaaaccttcg ggccccaaaa tgaagagaat tgggaacata ccccagctca cgaaaaacag
      301 ccatgccatg ccggtcacga cctggcggca ccgacccttt ggcacagtat gataaccctc
      361 gatatatgct ttggcggcgt gaaaaaatgt gttcgcgcca tagcacaatc caagacaaaa
      421 gaagatgact ttaacatagc cggttgccat ggcgctggta gccccccaca cgatagtccc
      481 gatgtctgag acaaggagtc ccatggttct cctgctgtag tcgttgctca ggccggtgag
      541 gttgctcagg cggataagga tgacaggaca agtgagcagc cactctgcat agcgcagcca
      601 ctgcacccgg tgtcctgtgg caaggtagag catagaggga ttcttaaact caaaaaagaa
      661 ctcgagaatc accttaacca tttcaatggc gcacacatag atctcctccc agccgcatgt
      721 agatttccag gtttggtagg catagaacat cagcagcaaa atgctgaatc ctgctgcaag
      781 ccactgcagg acatttgacg cggtctgagc gccgttcgtg ccgcgagatt caatccatcc
      841 ggcacagtaa cattgatcct cagggacc
//
