LOCUS       sequence-55127-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hughes RM, Bolger S, Tapadia H, Tucker CL
  TITLE     Light-mediated control of DNA transcription in yeast.
  JOURNAL   Methods. 2012 Aug 15.
  PUBMED    22922268
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2012 Aug 15."
COMMENT     Sequence Label: pRMH120
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             72..581
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 ccccaccaaa cccaaaaaaa gagatcttta atacgactca ctatagggcg agcgccgaag
       61 ctagcgccac catgaatgga gctataggag gtgacctttt gctcaatttt cctgacatgt
      121 cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt gattctcctc
      181 tcgccggctt ctttgccgat tcttcaatga ttaccggcgg cgagatggac agctatcttt
      241 cgactgccgg tttgaatctt ccgatgatgt acggtgagac gacggtggaa ggtgattcaa
      301 gactctcaat ttcgccggaa acgacgcttg ggactggaaa tttcaagaaa cggaagtttg
      361 atacagagac taaggattgt aatgagaaga agaagaagat gacgatgaac agagatgacc
      421 tagtagaaga aggagaagaa gagaagtcga aaataacaga gcaaaacaat gggagcacaa
      481 aaagcatcaa gaagatgaaa cacaaagcca agaaagaaga gaacaatttc tctaatgatt
      541 catctaaagt gacgaaggaa ttggagaaaa cggattatat tcatgttcgt gcacgacgag
      601 gccaagccac tgatagtcac agcatagcag aacgagttag aagagaaaag atcagtgaga
      661 gaatgaagtt tctacaagat ttggttcctg gatgcgacaa gatcacaggc aaagcaggga
      721 tgcttgatga aatcattaac tatgttcagt ctcttcagag acaaatcgag ttcttatcga
      781 tgaaactagc aattgtgaat ccaaggccgg attttgatat ggatgacatt tttgccaaag
      841 aggttgcctc aactccaatg actgtggtgc catctcctga aat
//
