LOCUS       sequence-55129-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: EGFP-Rab7A T22N
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..60
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(746..764)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(747..764)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(784..801)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    790..901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcggcatgg acgagctgta cnagggancc atgtacccat acgatgttcc ggattacgct
       61 tacccatacg atgttccgga ttacgctggt acctctagga agaaagtgtt gctgaaggtt
      121 atcatcctgg gagattctgg agtcgggaag aactcactca tgaaccagta tgtgaataag
      181 aaattcagca atcagtacaa agccacaata ggagctgact ttctgaccaa ggaggtgatg
      241 gtggatgaca ggctagtcac aatgcagata tgggacacag caggacagga acggttccag
      301 tctctcggtg tggccttcta cagaggtgca gactgctgcg ttctggtatt tgatgtgact
      361 gcccccaaca cattcaaaac cctagatagc tggagagatg agtttctcat ccaggccagt
      421 ccccgagatc ctgaaaactt cccatttgtt gtgttgggaa acaagattga cctcgaaaac
      481 agacaagtgg ccacaaagcg ggcacaggcc tggtgctaca gcaaaaacaa cattccctac
      541 tttgagacca gtgccaagga ggccatcaac gtggagcagg cgttccagac gattgcacgg
      601 aatgcactta agcaggaaac ggaggtggag ctgtacaacg aatttcctga acctatcaaa
      661 ctggacaaga atgaccgggc caaggcctcg gcagaaagct gcagttgcta agcggccgct
      721 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      781 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      841 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      901 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagc
//
