LOCUS       sequence-55134-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VP16-CIB1 for light-inducible protein expression when 
            coupled with GalBD-CRY2PHR or GalBD-CRY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Hughes RM, Bolger S, Tapadia H, Tucker CL
  TITLE     Light-mediated control of DNA transcription in yeast.
  JOURNAL   Methods. 2012 Aug 15.
  PUBMED    22922268
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2012 Aug 15."
COMMENT     Sequence Label: pRMH124-VP16-CIB1
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             150..170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             171..404
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc
       61 tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac
      121 aatcaactcc aagctttgca aagatgggcc ctaaaaagaa gcgtaaagtc gcccccccga
      181 ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg
      241 ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg
      301 gatttacccc ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg
      361 agcagatgtt taccgatgcc cttggaattg acgagtacgg tggggaattc ccggggatcc
      421 gtcgacgcgt ctgcagaagc ttcggcggca gcatgaatgg agctatagga ggtgaccttt
      481 tgctcaattt tcctgacatg tcggtcctag agcgccaaag ggctcacctc aagtacctca
      541 atcccacctt tgattctcct ctcgccggct tctttgccga ttcttcaatg attaccggcg
      601 gcgagatgga cagctatctt tcgactgccg gtttgaatct tccgatgatg tacggtgaga
      661 cgacggtgga aggtgattca agactctcaa tttcgccgga aacgacgctt gggactggaa
      721 atttcaagaa acggaagttt gatacagaga ctaaggattg taatgagaag aagaagaaga
      781 tgacgatgaa cagagatgac ctagtagaag aaggagaaga agagaagtcg aaaataacag
      841 agcaaaacaa tgggagcaca aaaagcatca agaagatgaa acacaaagcc aagaaagaag
      901 agaacaattt ctctaatgat tcatctaaag tgacgaagga
//
