LOCUS       sequence-55135-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW
  TITLE     Sds22 and Repo-Man stabilize chromosome segregation by counteracting
            Aurora B on anaphase kinetochores.
  JOURNAL   J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.
  PUBMED    22801782
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-07-23"; volume: "198(2)"; pages: "173-83. Epub 
            2012 Jul 16"
COMMENT     Sequence Label: pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(261..263,264..776)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(303..324)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(564..583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgaaattcct tttacaagac ggagatgaaa tcaagatcat ttgggataaa aacaataaat
       61 ttgtcattgg ctttaaagtg gaaattaacg atactacagg tctgtttaac gagggattag
      121 gtatgttaca agaacaaaga gtagtactta agcaaacagc cgaagaaaaa gatttggtga
      181 aaaagttagg aggatccggc ggcaaggtga acaagatcgt gaagaaccgc cgcaccgtgg
      241 ccatcggtgg ttccggagga gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      301 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      361 agggcgatgc cacctacggc aagctgaccc tgaagctgct ctgcaccacc ggcaagctgc
      421 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
      481 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      541 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      601 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      661 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
      721 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      781 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      841 tgctgcccga caaccactac ctgagctacc agtccgccct gttc
//
