LOCUS       sequence-55147-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral vector for reversible immortalization of 
            human primary cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-TERT-iresTK
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          572..637
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
       61 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
      121 acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt
      181 gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt
      241 tatgattata actgttatgc ctacttataa aggttacaga atatttttcc ataattttct
      301 tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat
      361 tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt
      421 ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat
      481 cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact
      541 gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta
      601 gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag
      661 gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag atccggggcc
      721 cactcataaa tccagttgcc gccacggtag ccaatcaccg tatcgtataa atcatcgtcg
      781 gtacgttcgg catcgctcat cacaatacgt gcctggacgt cgaggatttc gcgtgggtca
      841 atgccgcgcc agatccacat c
//
