LOCUS       sequence-55153-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Di Fiore B, Pines J
  TITLE     How cyclin A destruction escapes the spindle assembly checkpoint.
  JOURNAL   J Cell Biol. 2010 Aug 23;190(4):501-9.
  PUBMED    20733051
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-08-23"; volume: "190(4)"; pages: "501-9"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..196
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(237..542)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(339..358)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atccaccaca ctggactagt ggatccgagc tcggtaccaa
       61 gcttaaatcg aaccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata
      121 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      181 atatccagtc actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat
      241 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
      301 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
      361 cagctttcat ccccgatatg caccaccggg taaagttcac gggggacttt atctgacagc
      421 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
      481 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
      541 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
      601 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
      661 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
      721 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
      781 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
      841 acgcatactg ttatctggct tttagtaagc cggatcctct agattacgcc ccgcctgcca
      901 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
      961 ggcatgatga a
//
