LOCUS       sequence-55161-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Kristen Kwan
  TITLE     Kristen Kwan's plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 1055 pminTol R4-R3
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(113..236)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(256..272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(311..448,449..460)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(511..529)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(512..529)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(547..563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    571..587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(595..601,602..619,620..625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    640..661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
       61 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
      121 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
      181 tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca aagttgatag
      241 cttggcgtaa tcatggtcat agctgtttcc tgacgtcaac tcgagattac cctgttatcc
      301 ctacttcctt aatactcaag tacaatttta atggagtact tttttacttt tactcaagta
      361 agattctagc cagatacttt tacttttaat tgagtaaaat tttccctaag tacttgtact
      421 ttcacttgag taaaattttt gagtactttt tacacctctg ctcgaccata tgggagagct
      481 cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta
      541 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      601 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      661 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      721 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      841 ggcggtaata cggttatcca cagaa
//
