LOCUS       sequence-55178-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, 
            Munoz-Pinedo C, Green DR
  TITLE     Resistance to caspase-independent cell death requires persistence of
            intact mitochondria.
  JOURNAL   Dev Cell. 2010 May 18;18(5):802-13.
  PUBMED    20493813
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2010-05-18"; volume: "18(5)"; pages: "802-13"
COMMENT     Sequence Label: pcDNA3-Smac(1-60)mCherry
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(431..449)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     699..718
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccatggcggc tctgaagagt
      121 tggctgtcgc gcagcgtaac ttcattcttc aggtacagac agtgtttgtg tgttcctgtt
      181 gtggctaact ttaagaagcg gtgtttctca gaattgataa gaccatggca caaaactgtg
      241 acgattggct ttggagtaac cctgtgtgcg gttcctattg cagaattcgt gagcaagggc
      301 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      361 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      421 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      481 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      541 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      601 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      661 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      721 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      781 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      841 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      901 atcacctccc acaacgagga ctacaccatc gtgga
//
