LOCUS       sequence-55180-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, 
            Munoz-Pinedo C, Green DR
  TITLE     Resistance to caspase-independent cell death requires persistence of
            intact mitochondria.
  JOURNAL   Dev Cell. 2010 May 18;18(5):802-13.
  PUBMED    20493813
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2010-05-18"; volume: "18(5)"; pages: "802-13"
COMMENT     Sequence Label: pcDNA3-Smac(1-60)mCherry
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      293..302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(331..350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(332..350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(395..598)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(424..444)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        599..902
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
       61 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcacgaattc
      121 tgcaatagga accgcacaca gggttactcc aaagccaatc gtcacagttt tgtgccatgg
      181 tcttatcaat tctgagaaac accgcttctt aaagttagcc acaacaggaa cacacaaaca
      241 ctgtctgtac ctgaagaatg aagttacgct gcgcgacagc caactcttca gagccgccat
      301 ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
      361 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      421 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      481 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      541 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
      601 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
      661 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
      721 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
      781 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
      841 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
      901 cgcggaactc catatatggg ctatgaacta atgaccccgt a
//
