LOCUS       sequence-55182-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, 
            Munoz-Pinedo C, Green DR
  TITLE     Resistance to caspase-independent cell death requires persistence of
            intact mitochondria.
  JOURNAL   Dev Cell. 2010 May 18;18(5):802-13.
  PUBMED    20493813
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2010-05-18"; volume: "18(5)"; pages: "802-13"
COMMENT     Sequence Label: pcDNA3-Smac(1-60)mCherry
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..742)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(313..332)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     582..600
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttactt gtacagctcg tccatgccgc
       61 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      121 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
      181 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
      241 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
      301 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
      361 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
      421 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      481 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
      541 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
      601 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
      661 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
      721 tatcctcctc gcccttgctc acgaattctg caataggaac cgcacacagg gttactccaa
      781 agccaatcgt cacagttttg tgccatggtc ttatcaattc tgagaaacac cgcttcttaa
      841 agttagccac aacaggaaca cacaaacact gtctgtacct gaagaatgaa gttacgctgc
      901 gcgacagcca actcttcaga gcc
//
