LOCUS       sequence-55185-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Dubarry M, Loiodice I, Chen CL, Thermes C, Taddei A
  TITLE     Tight protein-DNA interactions favor gene silencing.
  JOURNAL   Genes Dev. 2011 Jul 1;25(13):1365-70.
  PUBMED    21724830
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2011-07-1"; volume: "25(13)"; pages: "1365-70"
COMMENT     Sequence Label: pAT222
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(66..86)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc cgctctagat
       61 taggcaacct ttctcttctt ctttggtgga gtacaggatc ccagctgcat taatgaatcg
      121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
      181 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
      241 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
      301 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
      361 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
      421 agcatcgcag tgggaacgat gccctcattc agcattagca tggtttgttg aaaaccggac
      481 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
      541 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
      601 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
      661 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
      721 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
      781 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
      841 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
      901 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggaggc a
//
