LOCUS       sequence-55194-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Azarin SM, Lian X, Larson EA, Popelka HM, de Pablo JJ, Palecek SP
  TITLE     Modulation of Wnt/beta-catenin signaling in human embryonic stem 
            cells using a 3-D microwell array.
  JOURNAL   Biomaterials. 2012 Mar;33(7):2041-9. Epub 2011 Dec 15.
  PUBMED    22177620
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomaterials."; date: "2012-03"; volume: "33(7)"; pages: "2041-9. 
            Epub 2011 Dec 15"
COMMENT     Sequence Label: pXL010-Wnt dual (GFP-Fire) reporter
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..100)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(242..907)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             complement(242..907)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat accggaaggg ccgggattct
       61 cctccacgtc accgcatgtt agaagacttc ctctgccctc accggtcaca ttgatcctag
      121 cagaagcaca ggctgcaggg tgacggtcca tcccgctctc ctgggcacaa gacatgggca
      181 gcgtgccatc atcctgcgcc gccaccgccg gcgggaagcc atggctgata tctcgagatc
      241 tggcgaaggc gatgggggtc ttgaaggcgt gctggtactc cacgatgccc agctcggtgt
      301 tgctgtgcag ctcctccacg cggcggaagg cgaacatggg gcccccgttc tgcaggatgc
      361 tggggtggat ggcgctcttg aagtgcatgt ggctgtccac cacgaagctg tagtagccgc
      421 cgtcgcgcag gctgaaggtg cgggcgaagc tgcccaccag cacgttatcg cccatggggt
      481 gcaggtgctc cacggtggcg ttgctgcgga tgatcttgtc ggtgaagatc acgctgtcct
      541 cggggaagcc ggtgcccacc accttgaagt cgccgatcac gcggccggcc tcgtagcggt
      601 agctgaagct cacgtgcagc acgccgccgt cctcgtactt ctcgatgcgg gtgttggtgt
      661 agccgccgtt gttgatggcg tgcaggaagg ggttctcgta gccgctgggg taggtgccga
      721 agtggtagaa gccgtagccc atcacgtggc tcagcaggta ggggctgaag gtcagggcgc
      781 ctttggtgct cttcatcttg ttggtcatgc ggccctgctc gggggtgccc tctccgccgc
      841 ccaccagctc gaactccacg ccgttcaggg tgccggtgat gcggcactcg atcttcatgg
      901 cgggcatggt ggctttacca acagtaccgg aatgccaagc tttttaccaa cagtaccgga
      961 atgcca
//
