LOCUS       sequence-55195-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72110-BBa_J72094
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     157..174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(207..234)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(250..321)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(573..857)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(631..650)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttattaatca gataaaatat ttctagaggc ctcccctgat tctgtggata accgggatct
       61 gtaaggatca accactttgt acaagaaagc tgggtcgaat tgattaccgc ctttgagtga
      121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      181 tgcacgtcga gttaggatcc tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      241 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      301 ctgagccttt cgttttattt gatgcctgga gatccttatt gtgagtctgg ctggttagtg
      361 gccatgagat attcgattgt gtcagtgaga tcatccaggt cgtcttgggt gatgcggtac
      421 tcctgattgg atatctttga gtagtgttca gcaatggcgc gggcagcgtc ggtttcggcg
      481 gggtctacag ataaagcgtt agagcaatgt ctaacgtcgt cgatggttgg tggaatgaaa
      541 gccataatta tgcctcactg taagatccgg gtatggagaa acagtagaga gttgcgataa
      601 aaagcgtcag gtaagatccg ctaatcttat ggataaaaat gctatggcat agcaaagtgt
      661 gacgccgtgc aaataatcaa tgtggacttt tctgccgtga ttatagacac ttttgttacg
      721 cgtttttgtc atggctttgg tcccgctttg ttacagaatg cttttaataa gcggggttac
      781 cggtttggtt agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata
      841 tggacaattg gtttcttctc tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa
      901 tgatcccctg ctgccgggat actcgtt
//
