LOCUS       sequence-55196-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Hammonds J, Ding L, Chu H, Geller K, Robbins A, Wang JJ, Yi H, 
            Spearman P
  TITLE     The tetherin/BST-2 coiled-coil ectodomain mediates plasma membrane 
            microdomain localization and restriction of particle release.
  JOURNAL   J Virol. 2012 Feb;86(4):2259-72. Epub 2011 Nov 30.
  PUBMED    22130541
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2012-02"; volume: "86(4)"; pages: "2259-72. Epub 2011 Nov 30"
COMMENT     Sequence Label: pFLAG-4S Tetherin
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(584..607)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(643..662)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(644..662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(707..806)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(736..756)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagtcactg cagcagagcg ctgaggccca
       61 gcagcacaat cagcagctgg ggcgccgcag cggagctgga gtcctgggag ctggggtagt
      121 acttcttgtc cgcgattctc acgctggaga cctggttttc tcttctgctt cgctccacct
      181 ctgcagacgc gtcctgaagt ttatggttta atgtagtgat ctctccctca agctcctcca
      241 ctttcttttg tccttgggcc ttctctgcat ccagggaagc cattagggcc atcacagtgt
      301 ggttgcaggt ggcggcctgg gcctcgctat cctgaaagcc cttctgggcc tcggtgctct
      361 cttgttgcag gagatgggtg acattgcgac actccatcac tgcccgaagg ccgtcccggc
      421 aggcctcgct gttggccttg atggtgaaga taatcaaggg cacccccaga atcacgatga
      481 tcaggagcac cagaattcct atccccagca gaagcttaca gcgcttatcc ccgtcttcca
      541 tgggcactct gcaatagtca tacgaagtag atgccatgga tcccttgtca tcgtcatcct
      601 tgtaatccat ggtgatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat
      661 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      721 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      781 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggg
//
